Skip navigation
Skip navigation

LRGUK-1 is required for basal body and Manchette function during spermatogenesis and male fertility

Liu, Yan; DeBoer, Kathleen; de Kretser, David M.; O’Donnell, Liza; O’Connor, Anne E.; Merriner, D. Jo; Okuda, Hidenobu; Whittle, Belinda; Jans, David A.; Efthymiadis, Athina; McLachlan, Robert I.; Ormandy, Christopher J.; Goodnow, Chris C.; Jamsai, Duangporn; O’Bryan, Moira K.

Description

Male infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head...[Show more]

dc.contributor.authorLiu, Yan
dc.contributor.authorDeBoer, Kathleen
dc.contributor.authorde Kretser, David M.
dc.contributor.authorO’Donnell, Liza
dc.contributor.authorO’Connor, Anne E.
dc.contributor.authorMerriner, D. Jo
dc.contributor.authorOkuda, Hidenobu
dc.contributor.authorWhittle, Belinda
dc.contributor.authorJans, David A.
dc.contributor.authorEfthymiadis, Athina
dc.contributor.authorMcLachlan, Robert I.
dc.contributor.authorOrmandy, Christopher J.
dc.contributor.authorGoodnow, Chris C.
dc.contributor.authorJamsai, Duangporn
dc.contributor.authorO’Bryan, Moira K.
dc.date.accessioned2015-07-17T00:19:18Z
dc.date.available2015-07-17T00:19:18Z
dc.identifier.issn1553-7404
dc.identifier.urihttp://hdl.handle.net/1885/14335
dc.description.abstractMale infertility affects at least 5% of reproductive age males. The most common pathology is a complex presentation of decreased sperm output and abnormal sperm shape and motility referred to as oligoasthenoteratospermia (OAT). For the majority of OAT men a precise diagnosis cannot be provided. Here we demonstrate that leucine-rich repeats and guanylate kinase-domain containing isoform 1 (LRGUK-1) is required for multiple aspects of sperm assembly, including acrosome attachment, sperm head shaping and the initiation of the axoneme growth to form the core of the sperm tail. Specifically, LRGUK-1 is required for basal body attachment to the plasma membrane, the appropriate formation of the sub-distal appendages, the extension of axoneme microtubules and for microtubule movement and organisation within the manchette. Manchette dysfunction leads to abnormal sperm head shaping. Several of these functions may be achieved in association with the LRGUK-1 binding partner HOOK2. Collectively, these data establish LRGUK-1 as a major determinant of microtubule structure within the male germ line.
dc.description.sponsorshipThis work was supported by grants from the NHMRC to (MKO, RIM, DMdK and CJO), the Australian Research Council (MKO, and CJO), the New South Wales Cancer Council (CJO), Cancer Institute New South Wales (CJO), Banque Nationale de Paris-Paribas Australia and New Zealand (CJO), RT Hall Trust (CJO), and the National Breast Cancer Foundation (CJO.) MKO, DAJ, RIM, CJO and CCG are the recipients of NHMRC Fellowships The Australian Phenomics Network is funded by the Australian Government through National Collaborative Research Infrastructure Strategy. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.publisherPublic Library of Science
dc.rights© 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.sourcePLOS Genetics
dc.titleLRGUK-1 is required for basal body and Manchette function during spermatogenesis and male fertility
dc.typeJournal article
local.identifier.citationvolume11
dcterms.dateAccepted2015-02-23
dc.date.issued2015-03-17
local.identifier.absfor060400 - GENETICS
local.identifier.ariespublicationa383154xPUB1341
local.publisher.urlhttps://www.plos.org/
local.type.statusPublished Version
local.contributor.affiliationWhittle, B., Australian Phenomics Facility, The Australian National University
local.identifier.essn1553-7404
local.bibliographicCitation.issue3
local.bibliographicCitation.startpagee1005090
local.bibliographicCitation.lastpage20
local.identifier.doi10.1371/journal.pgen.1005090
dc.date.updated2015-12-10T10:36:07Z
local.identifier.scopusID2-s2.0-84926311749
CollectionsANU Research Publications

Download

File Description SizeFormat Image
Liu et al LRGUK-1 is Required 2015.pdf15.29 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator