O-tert-Butyltyrosine, an NMR Tag for High-Molecular-Weight Systems and Measurements of Submicromolar Ligand Binding Affinities
O-tert-Butyltyrosine (Tby) is an unnatural amino acid that can be site-specifically incorporated into proteins using established orthogonal aminoacyl-tRNA synthetase/tRNA systems. Here we show that the tert-butyl group presents an outstanding NMR tag that can readily be observed in one-dimensional (1)H NMR spectra without any isotope labeling. Owing to rapid bond rotations and the chemical equivalence of the protons of a solvent-exposed tert-butyl group from Tby, the singlet resonance from the...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of the American Chemical Society|
|Chen et al O-tert-Butyltyrosine 2015.pdf||373.83 kB||Adobe PDF|
|Chen et al O-tert-Butyltyrosine 2015 Supporting Information.pdf||1.06 MB||Adobe PDF|
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