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O-tert-Butyltyrosine, an NMR Tag for High-Molecular-Weight Systems and Measurements of Submicromolar Ligand Binding Affinities

Chen, Wan-Na; Kuppan, Kekini Vahini; Lee, Michael David; Jaudzems, Kristaps; Otting, Gottfried; Huber, Thomas

Description

O-tert-Butyltyrosine (Tby) is an unnatural amino acid that can be site-specifically incorporated into proteins using established orthogonal aminoacyl-tRNA synthetase/tRNA systems. Here we show that the tert-butyl group presents an outstanding NMR tag that can readily be observed in one-dimensional (1)H NMR spectra without any isotope labeling. Owing to rapid bond rotations and the chemical equivalence of the protons of a solvent-exposed tert-butyl group from Tby, the singlet resonance from the...[Show more]

CollectionsANU Research Publications
Date published: 2015-03-19
Type: Journal article
URI: http://hdl.handle.net/1885/14319
Source: Journal of the American Chemical Society
DOI: 10.1021/jacs.5b01918

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