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Characterization of narrow-leaf lupin (Lupinus angustifolius L.) recombinant major allergen IgE-binding proteins and the natural β-conglutin counterparts in sweet lupin seed species

Jimenez-Lopez, Jose C; Foley, Rhonda C; Brear, Ella; Clarke, Victoria C; Lima-Cabello, Elena; Florido, Jose F; Singh, Karam B; Alché, Juan D; Smith, Penelope M C

Description

β-conglutin has been identified as a major allergen for Lupinus angustifolius seeds. The aim of this study was to evaluate the binding of IgE to five recombinant β-conglutin isoforms (rβ) that we overexpressed and purified and to their natural counterparts in different lupin species and cultivars. Western blotting suggested β-conglutins were the main proteins responsible for the IgE reactivity of the lupin species and cultivars. Newly identified polypeptides from "sweet lupin" may constitute a...[Show more]

CollectionsANU Research Publications
Date published: 2018-04-01
Type: Journal article
URI: http://hdl.handle.net/1885/139363
Source: Food chemistry
DOI: 10.1016/j.foodchem.2017.10.015

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