An epigenomic roadmap to induced pluripotency reveals DNA methylation as a reprogramming modulator
Lee, Dong-Sung; Shin, Jong-Yeon; Tonge, Peter D.; Puri, Mira C.; Lee, Seungbok; Park, Hansoo; Lee, Won-Chul; Hussein, Samer M. I.; Bleazard, Thomas; Yun, Ji-Young; Kim, Jihye; Li, Mira; Cloonan, Nicole; Wood, David; Clancy, Jennifer L.; Mosbergen, Rowland; Yi, Jae-Hyuk; Yang, Kap-Seok; Kim, Hyungtae; Rhee, Hwanseok; Wells, Christine A.; Preiss, Thomas; Grimmond, Sean M.; Rogers, Ian M.; Nagy, Andras; Seo, Jeong-Sun
Reprogramming of somatic cells to induced pluripotent stem cells involves a dynamic rearrangement of the epigenetic landscape. To characterize this epigenomic roadmap, we have performed MethylC-seq, ChIP-seq (H3K4/K27/K36me3) and RNA-Seq on samples taken at several time points during murine secondary reprogramming as part of Project Grandiose. We find that DNA methylation gain during reprogramming occurs gradually, while loss is achieved only at the ESC-like state. Binding sites of activated...[Show more]
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