Species identification using high resolution melting (HRM) analysis with random forest classification
Species identification is an important facet of forensic investigation. In this study, human and non-human species (cow, chicken, pig, sheep, cat, dog, rabbit, fox, kangaroo and wombat) were assayed on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) to rapidly screen for their species of origin using the high resolution melt (HRM) analysis targeting the 16S rRNA gene. Classification of HRM difference profiles using the onboard ViiA 7 software resulted in a classification accuracy...[Show more]
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