Osborn, Hannah L; Alonso-Cantabrana, Hugo; Sharwood, Robert E; Covshoff, Sarah; Evans, John R; Furbank, Robert; von Caemmerer, Susanne
In C₄ species, the major β-carbonic anhydrase (β-CA) localized in the mesophyll cytosol catalyses the hydration of CO₂ to HCO₃-, which phosphoenolpyruvate carboxylase uses in the first step of C₄ photosynthesis. To address the role of CA in C₄ photosynthesis, we generated transgenic Setaria viridis depleted in β-CA. Independent lines were identified with as little as 13% of wild-type CA. No photosynthetic defect was observed in the transformed lines at ambient CO₂ partial pressure (pCO₂). At...[Show more]
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