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Directed evolution of new and improved enzyme functions using an evolutionary intermediate and multidirectional search

Porter, Joanne L.; Boon, Priscilla L. S.; Murray, Tracy P.; Huber, Thomas; Collyer, Charles A.; Ollis, David L.


The ease with which enzymes can be adapted from their native roles and engineered to function specifically for industrial or commercial applications is crucial to enabling enzyme technology to advance beyond its current state. Directed evolution is a powerful tool for engineering enzymes with improved physical and catalytic properties and can be used to evolve enzymes where lack of structural information may thwart the use of rational design. In this study, we take the versatile and diverse α/β...[Show more]

CollectionsANU Research Publications
Date published: 2015-11-24
Type: Journal article
Source: ACS Chemical Biology
DOI: 10.1021/cb500809f


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