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The use of fluorescent target arrays for assessment of T cell responses in vivo

Quah, Benjamin J. C; Wijesundara, Danushka K; Ranasinghe, Charani; Parish, Christopher

Description

The ability to monitor T cell responses in vivo is important for the development of our understanding of the immune response and the design of immunotherapies. Here we describe the use of fluorescent target array (FTA) technology, which utilizes vital dyes such as carboxyfluorescein succinimidyl ester (CFSE), violet laser excitable dyes (CellTrace Violet: CTV) and red laser excitable dyes (Cell Proliferation Dye eFluor 670: CPD) to combinatorially label mouse lymphocytes into > 250 discernable...[Show more]

CollectionsANU Research Publications
Date published: 2014-06-19
Type: Journal article
URI: http://hdl.handle.net/1885/13715
Source: Journal of Visualized Experiments
DOI: 10.3791/51627

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