Protein Engineering of Escherichia coli β-glucuronidase
This thesis describes engineering studies with the Escherichia coli β-glucuronidase enzyme (E. coli β-GUS) that catalyzes the hydrolysis of D-glucuronic acids (glycone) that are conjugated through a β-O-glycosidic linkage to an aglycone. The enzyme is specific for the glucuronic acid component and will tolerate a variety of aglycones. A point mutation was known to convert β-GUS into a glucuronylsynthase, that is an enzyme capable of the synthesis of...[Show more]
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