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Protein Engineering of Escherichia coli β-glucuronidase

Lim, Jo Leen


This thesis describes engineering studies with the Escherichia coli β-glucuronidase enzyme (E. coli β-GUS) that catalyzes the hydrolysis of D-glucuronic acids (glycone) that are conjugated through a β-O-glycosidic linkage to an aglycone. The enzyme is specific for the glucuronic acid component and will tolerate a variety of aglycones. A point mutation was known to convert β-GUS into a glucuronylsynthase, that is an enzyme capable of the synthesis of...[Show more]

CollectionsOpen Access Theses
Date published: 2017
Type: Thesis (PhD)
DOI: 10.25911/5d6cf8147ddbb


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