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A peptide affinity reagent for isolating an intact and catalytically active multi-protein complex from mammalian cells

Saathoff, Hinnerk; Brofelth, Mattias; Trinh, Anne; Parker, Benjamin L; Ryan, Daniel P; Low, Jason; Webb, Sarah R; Silva, Ana P G; Mackay, Joel P; Shepherd, Nicholas E

Description

We have developed an approach for directly isolating an intact multi-protein chromatin remodeling complex from mammalian cell extracts using synthetic peptide affinity reagent 4. FOG1(1-15), a short peptide sequence known to target subunits of the nucleosome remodeling and deacetylase (NuRD) complex, was joined via a 35-atom hydrophilic linker to the StreptagII peptide. Loading this peptide onto Streptactin beads enabled capture of the intact NuRD complex from MEL cell nuclear extract. Gentle...[Show more]

CollectionsANU Research Publications
Date published: 2015-03-01
Type: Journal article
URI: http://hdl.handle.net/1885/12937
Source: Bioorganic & medicinal chemistry
DOI: 10.1016/j.bmc.2015.01.023

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