A peptide affinity reagent for isolating an intact and catalytically active multi-protein complex from mammalian cells
We have developed an approach for directly isolating an intact multi-protein chromatin remodeling complex from mammalian cell extracts using synthetic peptide affinity reagent 4. FOG1(1-15), a short peptide sequence known to target subunits of the nucleosome remodeling and deacetylase (NuRD) complex, was joined via a 35-atom hydrophilic linker to the StreptagII peptide. Loading this peptide onto Streptactin beads enabled capture of the intact NuRD complex from MEL cell nuclear extract. Gentle...[Show more]
|Collections||ANU Research Publications|
|Source:||Bioorganic & medicinal chemistry|
|1-s2.0-S0968089615000395-main.pdf||Main article pdf||1.55 MB||Adobe PDF|
|mmc1 (16).pdf||Supplementary Data||404.49 kB||Adobe PDF|
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