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Engineering herpes simplex viruses by infection–transfection methods including recombination site targeting by CRISPR/Cas9 nucleases

Russell, Tiffany A.; Stefanovic, Tijana; Tscharke, David C.


Herpes simplex viruses (HSVs) are frequent human pathogens and the ability to engineer these viruses underpins much research into their biology and pathogenesis. Often the ultimate aim is to produce a virus that has the desired phenotypic change and no additional alterations in characteristics. This requires methods that minimally disrupt the genome and, for insertions of foreign DNA, sites must be found that can be engineered without disrupting HSV gene function or expression. This study...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Journal of Virological Methods
DOI: 10.1016/j.jviromet.2014.11.009


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