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Construction of a bacterial artificial chromosome library, determination of genome size, and characterization of an Hsp70 gene family in Phytophthora nicotianae

Shan, Weixing; Hardham, Adrienne R

Description

The oomycete plant pathogen Phytophthora nicotianae causes diseases on a wide range of plant species. To facilitate isolation and functional characterization of pathogenicity genes, we have constructed a large-insert bacterial artificial chromosome (BAC) library using nuclear DNA from P. nicotianae H1111. The library contains 10,752 clones with an average insert size of 90kb and is free of mitochondrial DNA. The quality of the library was verified by hybridization with 37 genes, all of which...[Show more]

CollectionsANU Research Publications
Date published: 2004-03
Type: Journal article
URI: http://hdl.handle.net/1885/12208
Source: Fungal Genetics and Biology 41.3 (2004): 369–380
DOI: 10.1016/j.fgb.2003.11.012

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