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Production of monoclonal antibodies against peripheral-vesicle proteins in zoospores of Phytophthora nicotianae

Robold, A. V; Hardham, Adrienne R

Description

A coimmunisation protocol using microsomal fractions from Phytophthora nicotianae cells has enhanced the production of mono- clonal antibodies directed towards proteins produced during asexual sporulation. Over 40% of the antibodies targeted three categories of zoospore peripheral vesicles. Five antibodies label the contents of dorsal vesicles, with three of these reacting with two P. nicotianae polypeptides with a relative molecular mass of approximately 100 kDa. Two antibodies label the...[Show more]

CollectionsANU Research Publications
Date published: 2004-06
Type: Journal article
URI: http://hdl.handle.net/1885/12205
Source: Protoplasma 223. 2-4 (2004): 121–132
DOI: 10.1007/s00709-004-0042-x

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