Production of monoclonal antibodies against peripheral-vesicle proteins in zoospores of Phytophthora nicotianae
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Robold, A. V; Hardham, Adrienne R
Description
A coimmunisation protocol using microsomal fractions from Phytophthora nicotianae cells has enhanced the production of mono- clonal antibodies directed towards proteins produced during asexual sporulation. Over 40% of the antibodies targeted three categories of zoospore peripheral vesicles. Five antibodies label the contents of dorsal vesicles, with three of these reacting with two P. nicotianae polypeptides with a relative molecular mass of approximately 100 kDa. Two antibodies label the...[Show more]
Collections | ANU Research Publications |
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Date published: | 2004-06 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/12205 |
Source: | Protoplasma 223. 2-4 (2004): 121–132 |
DOI: | 10.1007/s00709-004-0042-x |
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