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Lipid binding activities of flax rust AvrM and AvrL567 effectors

Gan, Pamela H P; Rafiqi, Maryam; Ellis, Jeffrey G; Jones, David A; Hardham, Adrienne R; Dodds, Peter N

Description

Effectors are pathogen-encoded proteins that are thought to facilitate infection by manipulation of host cells. Evidence showing that the effectors of some eukaryotic plant pathogens are able to interact directly with cytoplasmic host proteins indicates that translocation of these proteins into host cells is an important part of infection. Recently, we showed that the flax rust effectors AvrM and AvrL567 are able to internalize into plant cells in the absence of the pathogen. Further,...[Show more]

dc.contributor.authorGan, Pamela H P
dc.contributor.authorRafiqi, Maryam
dc.contributor.authorEllis, Jeffrey G
dc.contributor.authorJones, David A
dc.contributor.authorHardham, Adrienne R
dc.contributor.authorDodds, Peter N
dc.date.accessioned2014-09-01T00:09:49Z
dc.date.available2014-09-01T00:09:49Z
dc.identifier.issn1559-2316
dc.identifier.urihttp://hdl.handle.net/1885/11984
dc.description.abstractEffectors are pathogen-encoded proteins that are thought to facilitate infection by manipulation of host cells. Evidence showing that the effectors of some eukaryotic plant pathogens are able to interact directly with cytoplasmic host proteins indicates that translocation of these proteins into host cells is an important part of infection. Recently, we showed that the flax rust effectors AvrM and AvrL567 are able to internalize into plant cells in the absence of the pathogen. Further, N-terminal sequences that were sufficient for uptake were identified for both these proteins. In light of the possibility that the internalization of fungal and oomycete effectors may require binding to specific phospholipids, the lipid binding activities of AvrM and AvrL567 mutants with different abilities to enter cells were tested. While AvrL567 was not found to bind to phospholipids, AvrM bound strongly to phosphatidyl inositol, phosphatidyl inositol monophosphates and phosphatidyl serine. However, a fragment of AvrM sufficient to direct uptake of a fusion protein into plant cells did not bind to these phospholipids. Thus, our results do not support the role of specific binding of AvrM and AvrL567 to phospholipids for uptake into the plant cytoplasm.
dc.format4 pages
dc.publisherLandes Bioscience
dc.rights© 2010 Landes Bioscience
dc.sourcePlant Signaling & Behavior 5. 10 (2010): 1272-1275
dc.subjecteffector
dc.subjectuptake
dc.subjecttranslocation
dc.subjectlipid
dc.subjectbinding
dc.subjectphosphatidylinositol
dc.titleLipid binding activities of flax rust AvrM and AvrL567 effectors
dc.typeJournal article
local.description.notesThis article is an addendum to Rafiqi, M. et. al. "Internalization of flax rust avirulence proteins into flax and tobacco cells can occur in the absence of the pathogen." The Plant Cell, 22.6 (June 2010): 2017-2032.
local.identifier.citationvolume5
dcterms.dateAccepted2010-07-07
dc.date.issued2010-10
local.identifier.absfor060704 - Plant Pathology
local.identifier.absfor069902 - Global Change Biology
local.identifier.ariespublicationu4956746xPUB20
local.publisher.urlhttps://www.landesbioscience.com/
local.type.statusPublished version
local.contributor.affiliationHardham, Adrienne R., Plant Science Division, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University
local.contributor.affiliationGan, Pamela H. P., Plant Science Division, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University
local.contributor.affiliationRafiqi, Maryam, Plant Science Division, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University
local.contributor.affiliationJones, David A., Plant Science Division, Research School of Biology, College of Medicine, Biology and Environment, The Australian National University
local.identifier.essn1559-2324
local.bibliographicCitation.issue10
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage4
local.identifier.doi10.4161/psb.5.10.13013
local.identifier.absseo829899 - Environmentally Sustainable Plant Production not elsewhere classified
local.identifier.absseo820209 - Ornamentals, Natives, Flowers and Nursery Plants
local.identifier.absseo820104 - Native Forests
dc.date.updated2015-12-08T03:12:55Z
local.identifier.scopusID2-s2.0-78449283258
CollectionsANU Research Publications

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