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Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues

Cheah, Mun Hon; Millar, A. Harvey; Myers, Ruth; Day, David; Roth, Justine; Hillier, Warwick; Badger, Murray


The reduction chemistry of molecular oxygen underpins the energy metabolism of multicellular organisms, liberating free energy needed to catalyse a plethora of enzymatic reactions. Measuring the isotope signatures of 16O and 18O during O2 reduction can provide insights into both kinetic and equilibrium isotope effects. However, current methods to measure O2 isotope signatures are time consuming and disruptive. This paper describes the application of membrane inlet mass spectrometry (MIMS) to...[Show more]

CollectionsANU Research Publications
Date published: 2014
Type: Journal article
Source: Analytical Chemistry 86. 10 (2014): 5171-5178
DOI: 10.1021/ac501086n


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