A study of substrate selectivity of aminoacyl-tRNA synthetases,
from both bacterial and eukaryotic organisms, using unnatural
amino acids is described and discussed in this thesis.
The first part of this thesis involved an investigation of the
substrate selectivity of bacterial (E. coli) aminoacyl-tRNA
synthetases, by protein synthesis using unnatural amino acids via
bacterial (E. coli) wild-type machinery. The levels of unnatural
amino acid...[Show more]
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