A Study of Protein Expression and Enzyme Selectivity Using Unnatural Amino Acids
A study of substrate selectivity of aminoacyl-tRNA synthetases, from both bacterial and eukaryotic organisms, using unnatural amino acids is described and discussed in this thesis. The first part of this thesis involved an investigation of the substrate selectivity of bacterial (E. coli) aminoacyl-tRNA synthetases, by protein synthesis using unnatural amino acids via bacterial (E. coli) wild-type machinery. The levels of unnatural amino acid...[Show more]
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