Bettadapura, J.; Addis, S. N. K.
Aims: The presence of a C-terminally extended form of NS1 (NS1’ protein) has been previously reported in encephalitic
flaviviruses, due to the presence of -1 programmed ribosomal frameshift at the N-terminal of NS2A protein. This present
study is aimed to further confirm that the NS1’ protein production is independent of the authentic cleavage at NS1-2A
Methodology and results: Six different constructs (P1-Leu, P2-Asp, P3-Phe, P3-Leu, P3-Gly and P5-8 Ala) containing
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