Engineering the glycoside hydrolase β-glucuronidase (β-GUS) to improve a non-native activity for the synthesis of O-glucuronides
The demand for O-glucuronides as potential therapeutic products and biomarkers continue to increase. However, large-scale synthesis of O glucuronides remains a challenge for the industry, and has prompted the development of an alternative synthetic routes. This dissertation extends from a previous enzyme engineering work that had introduced a site-specific mutation E504G in β glucuronidase (β-GUS), resulting in a functional glucuronylsynthase (Syn). However,...[Show more]
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