A study of enzyme catalysis of cycloaddition reactions
This thesis presents an investigation into the development of enzyme catalysis of cycloaddition reactions. Tyrosyl-tRNA synthetase was initially investigated as a catalyst for the cycloaddition between azides and alkynes and nitrile oxides and alkenes. From modeling studies, substrates were proposed based on the natural substrates of tyrosyltRNA synthetase. Each substrate was appended with either an azide, alkene, alkyne or nitrile oxide moiety. Tyrosyl-tRNA synthetase was expressed in E....[Show more]
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