Bacterial outer membrane vesicles induce plant immune responses
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Bahar, Ofir; Mordukhovich, Gideon; Luu, Dee Dee; Schwessinger, Benjamin; Daudi, Arsalan; Jehle, Anna Kristina; Felix, Georg; Ronald, Pamela C
Description
Gram-negative bacteria continuously pinch off portions of their outer membrane, releasing membrane vesicles. These outer membrane vesicles (OMVs) are involved in multiple processes including cell-to-cell communication, biofilm formation, stress tolerance, horizontal gene transfer, and virulence. OMVs are also known modulators of the mammalian immune response. Despite the well-documented role of OMVs in mammalian-bacterial communication, their interaction with plants is not well studied. To...[Show more]
dc.contributor.author | Bahar, Ofir | |
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dc.contributor.author | Mordukhovich, Gideon | |
dc.contributor.author | Luu, Dee Dee | |
dc.contributor.author | Schwessinger, Benjamin | |
dc.contributor.author | Daudi, Arsalan | |
dc.contributor.author | Jehle, Anna Kristina | |
dc.contributor.author | Felix, Georg | |
dc.contributor.author | Ronald, Pamela C | |
dc.date.accessioned | 2016-09-29T04:15:17Z | |
dc.date.available | 2016-09-29T04:15:17Z | |
dc.identifier.issn | 0894-0282 | |
dc.identifier.uri | http://hdl.handle.net/1885/109108 | |
dc.description.abstract | Gram-negative bacteria continuously pinch off portions of their outer membrane, releasing membrane vesicles. These outer membrane vesicles (OMVs) are involved in multiple processes including cell-to-cell communication, biofilm formation, stress tolerance, horizontal gene transfer, and virulence. OMVs are also known modulators of the mammalian immune response. Despite the well-documented role of OMVs in mammalian-bacterial communication, their interaction with plants is not well studied. To examine whether OMVs of plant pathogens modulate the plant immune response, we purified OMVs from four different plant pathogens and used them to treat Arabidopsis thaliana. OMVs rapidly induced a reactive oxygen species burst, medium alkalinization, and defense gene expression in A. thaliana leaf discs, cell cultures, and seedlings, respectively. Western blot analysis revealed that EF-Tu is present in OMVs and that it serves as an elicitor of the plant immune response in this form. Our results further show that the immune coreceptors BAK1 and SOBIR1 mediate OMV perception and response. Taken together, our results demonstrate that plants can detect and respond to OMV-associated molecules by activation of their immune system, revealing a new facet of plant-bacterial interactions. | |
dc.description.sponsorship | The work of O. Bahar was supported by postdoctoral award number FI-433-2010 from BARD, the United States–Israel Binational Agricultural Research and Development Fund. The work of B. Schwessinger was supported by a Human Frontier Science Program long-term postdoctoral fellowship (LT000674/2012) and a Discovery Early Career Award (DE150101897) from the Australian Research Council. The work at PCR lab was supported by a National Institutes of Health grant GM 59962. Work at the O. Bahar lab was supported by the German-Israeli Foundation for Scientific Research and Development (GIF), grant number I-2392-203.13/2015. Contribution number 558/16 from the Agricultural Research Organization, Volcani Center, Bet Dagan, Israel. | |
dc.publisher | American Phytopathological Society | |
dc.rights | © American Phytopathological Society | |
dc.source | Molecular plant-microbe interactions | |
dc.subject | arabidopsis | |
dc.subject | arabidopsis proteins | |
dc.subject | bacteria | |
dc.subject | cell membrane | |
dc.subject | gene expression regulation, plant | |
dc.subject | plant diseases | |
dc.title | Bacterial outer membrane vesicles induce plant immune responses | |
dc.type | Journal article | |
local.identifier.citationvolume | 29 | |
dc.date.issued | 2016-05 | |
local.publisher.url | http://www.apsnet.org/ | |
local.type.status | Published Version | |
local.contributor.affiliation | Schwessinger, B., Research School of Biology, The Australian National University | |
dc.relation | http://purl.org/au-research/grants/arc/DE150101897 | |
local.bibliographicCitation.issue | 5 | |
local.bibliographicCitation.startpage | 374 | |
local.bibliographicCitation.lastpage | 384 | |
local.identifier.doi | 10.1094/MPMI-12-15-0270-R | |
Collections | ANU Research Publications |
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