Targeted Knockdown of GDCH in Rice Leads to a Photorespiratory-Deficient Phenotype Useful as a Building Block for C₄ Rice
Lin, HsiangChun; Karki, Shanta; Coe, Robert A; Bagha, Shaheen; Khoshravesh, Roxana; Balahadia, C Paolo; Ver Sagun, Julius; Tapia, Ronald; Israel, W Krystler; Montecillo, Florencia; de Luna, Albert; Danila, Florence R; Lazaro, Andrea; Realubit, Czarina M; Acoba, Michelle G; Sage, Tammy L; von Caemmerer, Susanne; Furbank, Robert T; Cousins, Asaph B; Hibberd, Julian M; Quick, W Paul; Covshoff, Sarah
The glycine decarboxylase complex (GDC) plays a critical role in the photorespiratory C₂ cycle of C₃ species by recovering carbon following the oxygenation reaction of ribulose-1,5-bisphosphate carboxylase/oxygenase. Loss of GDC from mesophyll cells (MCs) is considered a key early step in the evolution of C₄ photosynthesis. To assess the impact of preferentially reducing GDC in rice MCs, we decreased the abundance of OsGDCH (Os10g37180) using an artificial microRNA (amiRNA) driven by a promoter...[Show more]
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|Source:||Plant & cell physiology|
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