Skip navigation
Skip navigation

Synthesis of a Cytokinin Linked by a Spacer to Dexamethasone and Biotin: Conjugates to Detect Cytokinin-Binding Proteins

Wang, You; Letham, David S; John, Peter C L; Zhang, Ren

Description

Yeast cells expressing cDNA libraries have provided two new approaches to facilitate further identification of cytokinin-binding proteins and receptors. These are the yeast three hybrid (Y3H) system and fluorescence activated cell sorting (FACS). The Y3H system requires a synthetic hybrid ligand comprising an "anchor" moiety (e.g., dexamethasone) linked to a cytokinin via a spacer. In the yeast nucleus, this ligand by binding connects two fusion proteins leading to a reporter gene activation...[Show more]

CollectionsANU Research Publications
Date published: 2016-04-30
Type: Journal article
URI: http://hdl.handle.net/1885/108602
Source: Molecules
DOI: 10.3390/molecules21050576

Download

File Description SizeFormat Image
01_Wang_Synthesis_of_a_Cytokinin_2016.pdf1.19 MBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  20 July 2017/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator