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Synthesis of a Cytokinin Linked by a Spacer to Dexamethasone and Biotin: Conjugates to Detect Cytokinin-Binding Proteins

Wang, You; Letham, David S; John, Peter C L; Zhang, Ren


Yeast cells expressing cDNA libraries have provided two new approaches to facilitate further identification of cytokinin-binding proteins and receptors. These are the yeast three hybrid (Y3H) system and fluorescence activated cell sorting (FACS). The Y3H system requires a synthetic hybrid ligand comprising an "anchor" moiety (e.g., dexamethasone) linked to a cytokinin via a spacer. In the yeast nucleus, this ligand by binding connects two fusion proteins leading to a reporter gene activation...[Show more]

CollectionsANU Research Publications
Date published: 2016-04-30
Type: Journal article
Source: Molecules
DOI: 10.3390/molecules21050576
Access Rights: Open Access


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