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Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods

Gyawali, P; Ahmed, W; Sidhu, J P S; Nery, S V; Clements, A. C.; Traub, R; McCarthy, J. S.; Llewellyn, S; Jagals, P; Toze, S


In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction (P < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. A reduction...[Show more]

CollectionsANU Research Publications
Date published: 2016-06-15
Type: Journal article
Source: Environmental science and pollution research international
DOI: 10.1007/s11356-016-7039-9


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