Skip navigation
Skip navigation

High yield production of a soluble human interleukin-3 variant from E. coli with wild-type bioactivity and improved radiolabeling properties

Hercus, Timothy R; Barry, Emma F; Dottore, Mara; McClure, Barbara J; Webb, Andrew I; Lopez, Angela F; Young, Ian G; Murphy, James M

Description

Human interleukin-3 (hIL-3) is a polypeptide growth factor that regulates the proliferation, differentiation, survival and function of hematopoietic progenitors and many mature blood cell lineages. Although recombinant hIL-3 is a widely used laboratory reagent in hematology, standard methods for its preparation, including those employed by commercial suppliers, remain arduous owing to a reliance on refolding insoluble protein expressed in E. coli. In addition, wild-type hIL-3 is a poor...[Show more]

CollectionsANU Research Publications
Date published: 2013-08-26
Type: Journal article
URI: http://hdl.handle.net/1885/10607
Source: PLoS One 8.8 (2013): e74376
DOI: 10.1371/journal.pone.0074376

Download

File Description SizeFormat Image
HercusHighyieldproduction2013.pdf866.92 kBAdobe PDFThumbnail


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  23 August 2018/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator