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Chemical composition and antimicrobial activity of honeybee (Apis mellifera ligustica) propolis from subtropical eastern Australia

Massaro, Carmelina Flavia; Simpson, Jack; Powell, Daniel; Brooks, Peter

Description

Propolis is a material manufactured by bees and contains beeswax, bee salivary secretions and plant resins. Propolis preparations have been used for millennia by humans in food, cosmetics and medicines due to its antibacterial effects. Within the hive, propolis plays an important role in bees' health, with much of its bioactivity largely dependent on the plant resins the bees select for its production. Few chemical studies are available on the chemistry of propolis produced by Australian...[Show more]

dc.contributor.authorMassaro, Carmelina Flavia
dc.contributor.authorSimpson, Jack
dc.contributor.authorPowell, Daniel
dc.contributor.authorBrooks, Peter
dc.date.accessioned2016-06-14T23:21:45Z
dc.identifier.issn0028-1042
dc.identifier.urihttp://hdl.handle.net/1885/104076
dc.description.abstractPropolis is a material manufactured by bees and contains beeswax, bee salivary secretions and plant resins. Propolis preparations have been used for millennia by humans in food, cosmetics and medicines due to its antibacterial effects. Within the hive, propolis plays an important role in bees' health, with much of its bioactivity largely dependent on the plant resins the bees select for its production. Few chemical studies are available on the chemistry of propolis produced by Australian honeybees (Apis mellifera, Apidae). This study aimed to determine the chemical composition as well as in vitro antimicrobial effects of propolis harvested from honeybees in subtropical eastern Australia. Honeybee propolis was produced using plastic frames and multiple beehives in two subtropical sites in eastern Australia. Methanolic extracts of propolis were analysed by liquid chromatography with ultraviolet detection and high-resolution mass spectrometry (ultra-high-pressure liquid chromatography (UHPLC)-UV-high-resolution tandem mass spectrometry (HR-MS/MS)) and by gas chromatography mass spectrometry (GC-MS). The resulting chemical data were dereplicated for compound characterisation. The two crude extracts in abs. ethanol were tested in vitro by the agar diffusion and broth dilution methods, using a phenol standard solution as the positive control and abs. ethanol as the negative control. Chemical constituents were identified to be pentacyclic triterpenoids and C-prenylated flavonoids, including Abyssinoflavanone VII, Propolin C and Nymphaeol C. The two propolis crude extracts showed bactericidal effects at the minimal inhibitory concentrations of 0.37-2.04 mg mL(-1) against Staphylococcus aureus ATCC 25923. However, the extracts were inactive against Klebsiella pneumoniae ATCC 13883 and Candida albicans ATCC 10231. The antistaphylococcal potential of propolis was discussed, also in relation to honeybees' health, as it warrants further investigations on the social and individual immunities of Australian honeybees.
dc.publisherSpringer
dc.rightsThis journal has changed it's name to 'The Science of Nature'.
dc.sourceNaturwissenschaften
dc.titleChemical composition and antimicrobial activity of honeybee (Apis mellifera ligustica) propolis from subtropical eastern Australia
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume102
dc.date.issued2015
local.identifier.absfor060899 - Zoology not elsewhere classified
local.identifier.ariespublicationu9511635xPUB1525
local.type.statusPublished Version
local.contributor.affiliationMassaro, Carmelina Flavia, University of the Sunshine Coast
local.contributor.affiliationSimpson, Jack, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPowell, Daniel, University of the Sunshine Coast
local.contributor.affiliationBrooks, Peter, University of the Sunshine Coast
local.description.embargo2037-12-31
local.bibliographicCitation.issue68
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage11
local.identifier.doi10.1007/s00114-015-1318-z
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-06-14T09:20:05Z
CollectionsANU Research Publications

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