Leaf rolling allows quantification of mRNA abundance in mesophyll cells of sorghum
In the leaves of most C4 plants, mesophyll (M) and bundle sheath (BS) cells develop and maintain highly differentiated biochemical networks. Separation and analysis of M and BS cells has greatly influenced our understanding of the C4 pathway. A number of approaches including mechanical separation, digestion with cell wall degrading cocktails, laser-capture microdissection, and leaf rolling have been used to isolate these cell types. Although leaf rolling is conceptually and practically the...[Show more]
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|Source:||Journal of Experimental Botany|
|01_Covshoff_Leaf_rolling_allows_2013.pdf||2.94 MB||Adobe PDF||Request a copy|
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