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Leaf rolling allows quantification of mRNA abundance in mesophyll cells of sorghum

Covshoff, Sarah; Furbank, Robert; Leegood, Richard C; Hibberd, Julian


In the leaves of most C4 plants, mesophyll (M) and bundle sheath (BS) cells develop and maintain highly differentiated biochemical networks. Separation and analysis of M and BS cells has greatly influenced our understanding of the C4 pathway. A number of approaches including mechanical separation, digestion with cell wall degrading cocktails, laser-capture microdissection, and leaf rolling have been used to isolate these cell types. Although leaf rolling is conceptually and practically the...[Show more]

CollectionsANU Research Publications
Date published: 2013
Type: Journal article
Source: Journal of Experimental Botany
DOI: 10.1093/jxb/ers286


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