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Analytical bias in the measurement of serum 25-hydroxyVitamin D concentrations impairs assessment of Vitamin D status in clinical and research settings

Black, Lucinda; Anderson, Denise; Clarke, Michael; Ponsonby, Anne-Louise; Chapman, C; Coulthard, Alan; Dear, Keith; Dwyer, Terry; Kilpatrick, Trevor J; Lucas, Robyn

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Measured serum 25-hydroxyvitamin D concentrations vary depending on the type of assay used and the specific laboratory undertaking the analysis, impairing the accurate assessment of vitamin D status. We investigated differences in serum 25-hydroxyvitamin D concentrations measured at three laboratories (laboratories A and B using an assay based on liquid chroma-tography-tandem mass spectrometry and laboratory C using a DiaSorin Liaison assay), against a laboratory using an assay based on liquid...[Show more]

dc.contributor.authorBlack, Lucinda
dc.contributor.authorAnderson, Denise
dc.contributor.authorClarke, Michael
dc.contributor.authorPonsonby, Anne-Louise
dc.contributor.authorChapman, C
dc.contributor.authorCoulthard, Alan
dc.contributor.authorDear, Keith
dc.contributor.authorDwyer, Terry
dc.contributor.authorKilpatrick, Trevor J
dc.contributor.authorLucas, Robyn
dc.date.accessioned2016-06-14T23:20:17Z
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1885/103293
dc.description.abstractMeasured serum 25-hydroxyvitamin D concentrations vary depending on the type of assay used and the specific laboratory undertaking the analysis, impairing the accurate assessment of vitamin D status. We investigated differences in serum 25-hydroxyvitamin D concentrations measured at three laboratories (laboratories A and B using an assay based on liquid chroma-tography-tandem mass spectrometry and laboratory C using a DiaSorin Liaison assay), against a laboratory using an assay based on liquid chromatography-tandem mass spectrome-try that is certified to the standard reference method developed by the National Institute of Standards and Technology and Ghent University (referred to as the 'certified laboratory'). Separate aliquots from the same original serum sample for a subset of 50 participants from the Ausim-mune Study were analysed at the four laboratories. Bland-Altman plots were used to visually check agreement between each laboratory against the certified laboratory. Compared with the certified laboratory, serum 25-hydroxyvitamin D concentrations were on average 12.4 nmol/L higher at laboratory A (95% limits of agreement: -17.8,42.6); 12.8 nmol/L higher at laboratory B (95% limits of agreement: 0.8,24.8); and 10.6 nmol/L lower at laboratory C (95% limits of agreement: -484,27.1). The prevalence of vitamin D deficiency (defined here as 25-hydroxyvitamin D <50 nmol/L) was 24%, 16%, 12% and 41% at the certified laboratory, and laboratories A, B, and C, respectively. Our results demonstrate considerable differences in the measurement of 25-hydroxyvitamin D concentrations compared with a certified laboratory, even between laboratories using assays based on liquid chromatography-tandem mass spectrometry, which is often considered the gold-standard assay. To ensure accurate and reliable measurement of serum 25-hydroxyvitamin D concentrations, all laboratories should use an accuracy-based quality assurance system and, ideally, comply with international standardisation efforts.
dc.publisherPublic Library of Science
dc.rightsAuthor/s retain copyright
dc.sourcePLOS ONE (Public Library of Science)
dc.titleAnalytical bias in the measurement of serum 25-hydroxyVitamin D concentrations impairs assessment of Vitamin D status in clinical and research settings
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume10
dc.date.issued2015
local.identifier.absfor110300 - CLINICAL SCIENCES
local.identifier.absfor111700 - PUBLIC HEALTH AND HEALTH SERVICES
local.identifier.ariespublicationU3488905xPUB6142
local.type.statusPublished Version
local.contributor.affiliationBlack, Lucinda, Telethon Kids Institute
local.contributor.affiliationAnderson, Denise, Telethon Kids Institute
local.contributor.affiliationClarke, Michael, University of Western Australia
local.contributor.affiliationPonsonby, Anne-Louise, Murdoch Children's Research Institute
local.contributor.affiliationLucas, Robyn, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChapman, C, Barwon Health
local.contributor.affiliationCoulthard, Alan, Royal Brisbane and Women's Hospital
local.contributor.affiliationDear, Keith, Duke Kunshan University
local.contributor.affiliationDwyer, Terry, Murdoch Children's Research Institute
local.contributor.affiliationKilpatrick, Trevor J, University of Melbourne
local.bibliographicCitation.issue8
local.identifier.doi10.1371/journal.pone.0135478
dc.date.updated2016-06-14T08:47:28Z
local.identifier.scopusID2-s2.0-84942874244
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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