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In vivo assessment of rodent Plasmodium parasitemia and merozoite invasion by flow cytometry

Lelliott, Patrick; McMorran, Brendan; Foote, Simon; Burgio, Gaetan

Description

During blood stage infection, malaria parasites invade, mature, and replicate within red blood cells (RBCs). This results in a regular growth cycle and an exponential increase in the proportion of malaria infected RBCs, known as parasitemia. We describe a flow cytometry based protocol which utilizes a combination of the DNA dye Hoechst, and the mitochondrial membrane potential dye, JC-1, to identify RBCs which contain parasites and therefore the parasitemia, of in vivo blood samples from...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
URI: http://hdl.handle.net/1885/103268
Source: Journal of Visualized Experiments
DOI: 10.3791/52736

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