Palmer, William M; Martin, Antony P; Flynn, Jamie R; Reed, Stephanie L.; White, Rosemary Gillian; Furbank, Robert; Grof, C.P.L.
Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins and IgG-antibodies in Tobacco and Arabidopsis leaves. Enzyme treatment enabled penetration of antibodies into whole tissues without the need for any sectioning of the material, thus facilitating...[Show more]
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