Translational Incorporation of Unsaturated Amino Acids
In nature proteins are synthesised using twenty standard proteinogenic amino acids. The unique linear sequences of these monomeric building blocks dictate the structure and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due to the high fidelity of aminoacyl-tRNA synthetases. This thesis is concerned with the in vitro residue-specific incorporation of unnatural amino acids to produce...[Show more]
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