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Changes in Dendritic Axial Resistance Alter Synaptic Integration in Cerebellar Purkinje Cells

Bekkers, John M.

Description

The ability of neurons to process synaptic inputs depends critically on their passive electrical properties. The intracellular resistivity, R(i), is one of the parameters that determine passive properties, yet few experiments have explored how changes in R(i) might affect synaptic integration. In this work, I addressed this issue by using targeted dendritic occlusion to locally increase R(i) in cerebellar Purkinje cells and examining the consequences of this manipulation for the summation of...[Show more]

dc.contributor.authorBekkers, John M.
dc.date.accessioned2016-03-24T00:38:02Z
dc.date.available2016-03-24T00:38:02Z
dc.identifier.issn0006-3495
dc.identifier.urihttp://hdl.handle.net/1885/100878
dc.description.abstractThe ability of neurons to process synaptic inputs depends critically on their passive electrical properties. The intracellular resistivity, R(i), is one of the parameters that determine passive properties, yet few experiments have explored how changes in R(i) might affect synaptic integration. In this work, I addressed this issue by using targeted dendritic occlusion to locally increase R(i) in cerebellar Purkinje cells and examining the consequences of this manipulation for the summation of synaptic inputs. To achieve dendritic occlusion, I used two glass micropipettes to gently pinch the dendritic trunk close to the soma. This pinching produced stereotypical changes in the responses to test pulses applied at the soma under voltage and current clamp. A simple model confirmed that these changes were due to increases in R(i) in the dendritic trunk. These localized increases in R(i) produced striking alterations in the shapes of postsynaptic potentials at the soma, increasing their amplitude and accelerating their decay kinetics. As a consequence, dendritic occlusion sharpened temporal precision during the summation of synaptic inputs. These findings highlight the importance of local changes in intracellular resistivity for the passive electrical properties of neurons, with implications for their ability to process synaptic information.
dc.description.sponsorshipThis work was begun in the laboratory of Michael Ha¨usser (University College London) with the support of a short-term travel grant from the Wellcome Trust. Subsequent funding was provided by the John Curtin School of Medical Research.
dc.publisherBiophysical Society
dc.rights© 2011 by the Biophysical Society. http://www.sherpa.ac.uk/romeo/issn/0006-3495/..."Author's post-print on non-commercial hosting platforms including institutional repositories. 12 months embargo." from SHERPA/RoMEO site (as at 24/03/16).
dc.sourceBiophysical Journal
dc.subjectanimals
dc.subjectdendrites
dc.subjectfemale
dc.subjectglass
dc.subjectmale
dc.subjectpressure
dc.subjectpurkinje cells
dc.subjectrats
dc.subjectrats, wistar
dc.subjectsynapses
dc.subjectsynaptic potentials
dc.subjectmodels, biological
dc.titleChanges in Dendritic Axial Resistance Alter Synaptic Integration in Cerebellar Purkinje Cells
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume100
dc.date.issued2011
local.identifier.absfor110902
local.identifier.ariespublicationf2965xPUB1987
local.publisher.urlhttp://www.biophysics.org/
local.type.statusAccepted Version
local.contributor.affiliationBekkers, John, College of Medicine, Biology and Environment, CMBE John Curtin School of Medical Research, Eccles Institute of Neuroscience, The Australian National University
local.identifier.essn1542-0086
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage1198
local.bibliographicCitation.lastpage1206
local.identifier.doi10.1016/j.bpj.2011.01.042
dc.date.updated2016-06-14T08:35:02Z
local.identifier.scopusID2-s2.0-79953836215
local.identifier.thomsonID000288049400007
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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