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Facile mutant identification via a single parental backcross method and application of whole genome sequencing based mapping pipelines

Allen, Robert S.; Nakasugi, Kenlee; Doran, Rachel L.; Millar, Anthony A.; Waterhouse, Peter M.

Description

Forward genetic screens have identified numerous genes involved in development and metabolism, and remain a cornerstone of biological research. However, to locate a causal mutation, the practice of crossing to a polymorphic background to generate a mapping population can be problematic if the mutant phenotype is difficult to recognize in the hybrid F2 progeny, or dependent on parental specific traits. Here in a screen for leaf hyponasty mutants, we have performed a single backcross of an Ethane...[Show more]

CollectionsANU Research Publications
Date published: 2013-09-13
Type: Journal article
URI: http://hdl.handle.net/1885/100181
Source: Frontiers in Plant Science
DOI: 10.3389/fpls.2013.00362

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