Facile mutant identification via a single parental backcross method and application of whole genome sequencing based mapping pipelines
Forward genetic screens have identified numerous genes involved in development and metabolism, and remain a cornerstone of biological research. However, to locate a causal mutation, the practice of crossing to a polymorphic background to generate a mapping population can be problematic if the mutant phenotype is difficult to recognize in the hybrid F2 progeny, or dependent on parental specific traits. Here in a screen for leaf hyponasty mutants, we have performed a single backcross of an Ethane...[Show more]
|Collections||ANU Research Publications|
|Source:||Frontiers in Plant Science|
|01_Allen_Facile_mutant_identification_2013.pdf||Published Version||1.45 MB||Adobe PDF|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.