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Expression, purification, crystallization and preliminary X-ray diffraction analysis of chloride intracellular channel 2 (CLIC2)

Cromer, Brett A; Gorman, Michael A; Hansen, Guido; Adams, Julian J; Coggan, Marjorie; Board, Philip; Parker, Michael William

Description

The chloride intracellular channel (CLIC) family of proteins are unusual in that they can exist in either an integral membrane-channel form or a soluble form. Here, the expression, purification, crystallization and preliminary diffraction analysis of CLIC2, one of the least-studied members of this family, are reported. Human CLIC2 was crystallized in two different forms, both in the presence of reduced glutathione and both of which diffracted to better than 1.9 Å resolution. Crystal form A...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
URI: http://hdl.handle.net/10440/605
http://digitalcollections.anu.edu.au/handle/10440/605
Source: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
DOI: 10.1107/S1744309107049159

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