Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis
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Ioannou, Charikleia; Schaeffer, Patrick; Dixon, Nicholas; Soultanas, Panos
Description
The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn2+-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn2+. DnaI binds ATP and exhibits ATPase activity that is...[Show more]
Collections | ANU Research Publications |
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Date published: | 2006-09-26 |
Type: | Journal article |
URI: | http://hdl.handle.net/10440/538 http://digitalcollections.anu.edu.au/handle/10440/538 |
Source: | Nucleic Acids Research |
DOI: | 10.1093/nar/gkl690 |
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Ioannou_Helicase2006.pdf | 2.09 MB | Adobe PDF | ![]() |
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