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Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis

Ioannou, Charikleia; Schaeffer, Patrick; Dixon, Nicholas; Soultanas, Panos


The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn2+-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn2+. DnaI binds ATP and exhibits ATPase activity that is...[Show more]

CollectionsANU Research Publications
Date published: 2006-09-26
Type: Journal article
Source: Nucleic Acids Research
DOI: 10.1093/nar/gkl690


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