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GM-CSF promoter chromatin remodelling and gene transcription display distinct signal and transcription factor requirements

Brettingham-Moore, Kate H; Rao, Sudha; Juelich, Torsten; Shannon, M Frances; Holloway, Adele F

Description

Granulocyte-macrophage colony stimulating factor (GM-CSF) plays a key role in myeloid cell function and is rapidly and transiently expressed in T cells in response to immune or inflammatory stimuli. Induction of GM-CSF gene expression is accompanied by changes in chromatin structure across the proximal promoter region of the gene. We show that the promoter remodelling and subsequent gene transcription occurs with distinct signal and transcription factor requirements. Activation of the protein...[Show more]

dc.contributor.authorBrettingham-Moore, Kate H
dc.contributor.authorRao, Sudha
dc.contributor.authorJuelich, Torsten
dc.contributor.authorShannon, M Frances
dc.contributor.authorHolloway, Adele F
dc.date.accessioned2009-06-23T03:46:24Z
dc.date.accessioned2010-12-20T06:04:33Z
dc.date.available2009-06-23T03:46:24Z
dc.date.available2010-12-20T06:04:33Z
dc.identifier.citationNucleic Acids Research 33.1 (2005): 225-234
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttp://hdl.handle.net/10440/519
dc.identifier.urihttp://digitalcollections.anu.edu.au/handle/10440/519
dc.description.abstractGranulocyte-macrophage colony stimulating factor (GM-CSF) plays a key role in myeloid cell function and is rapidly and transiently expressed in T cells in response to immune or inflammatory stimuli. Induction of GM-CSF gene expression is accompanied by changes in chromatin structure across the proximal promoter region of the gene. We show that the promoter remodelling and subsequent gene transcription occurs with distinct signal and transcription factor requirements. Activation of the protein kinase C (PKC) signalling pathway is sufficient to induce changes in chromatin structure across the promoter, but both the PKC and calcium signalling pathways are required for efficient gene transcription. Although NFAT transcription factors contribute to GM-CSF gene transcription, they are not required for promoter remodelling. However, the presence of the nuclear factor-B transcription factor, c-Rel, in the nucleus is strongly correlated with and required for the events of chromatin remodelling.
dc.format10 pages
dc.publisherOxford University Press
dc.rights"The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org." - from article
dc.sourceNucleic Acids Research
dc.source.urihttp://nar.oxfordjournals.org/cgi/reprint/33/1/225
dc.source.urihttp://nar.oxfordjournals.org/cgi/content/full/33/1/225
dc.subjectKeywords: granulocyte macrophage colony stimulating factor; immunoglobulin enhancer binding protein; protein kinase C; transcription factor; transcription factor NFAT; transcription factor Rel; animal cell; animal tissue; article; bone marrow cell; calcium signalin
dc.titleGM-CSF promoter chromatin remodelling and gene transcription display distinct signal and transcription factor requirements
dc.typeJournal article
local.description.refereedYes
local.identifier.citationvolume33
dcterms.dateAccepted2004-12-13
dc.date.issued2005-01-12
local.identifier.absfor110706
local.identifier.ariespublicationMigratedxPub16658
local.type.statusPublished Version
local.contributor.affiliationBrettingham-Moore, Kate H, University of Tasmania
local.contributor.affiliationRao, Sudha, John Curtin School of Medical Research, Division of Immunology and Genetics
local.contributor.affiliationJuelich, Torsten, John Curtin School of Medical Research, Division of Immunology and Genetics
local.contributor.affiliationShannon, M Frances, John Curtin School of Medical Research, Division of Molecular Bioscience
local.contributor.affiliationHolloway, Adele F, University of Tasmania
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage225
local.bibliographicCitation.lastpage234
local.identifier.doi10.1093/nar/gki161
dc.date.updated2015-12-12T08:23:27Z
local.identifier.scopusID2-s2.0-13744258909
CollectionsANU Research Publications

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