Quantification of Thrombus Kinetics Through Inhibiting Receptor Shedding with Label-Free Imaging Flow Assay

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dc.contributor.authorZheng, Yujieen
dc.contributor.authorMontague, Samantha J.en
dc.contributor.authorLim, Yean J.en
dc.contributor.authorGardiner, Elizabeth E.en
dc.contributor.authorLee, Woei Mingen
dc.coverage.spatialSan Diegoen
dc.date.accessioned2025-06-24T01:35:25Z
dc.date.available2025-06-24T01:35:25Z
dc.date.issued2021en
dc.description.abstractIn this paper, we use a high resolution multimodality label-free flow imaging assay to investigate the effect of platelet shedding of key adheso-signalling receptors in thrombus formation assay by inhibiting activation-induced metalloproteolytic cleavage of platelet receptors (GPIb of the GPIb-IX-V complex and GPVI).en
dc.description.sponsorshipUsing COSI, we unpicks intra-thrombus mass and adhesive platelet-only activity events under fluid shear stress. With a broad range metalloproteinase inhibitor blocking shedding of platelet adhesion receptors, we shown the elevated adhesive platelet activity at average of 42.1 µm/s and minimal change in intra-thrombus mass. ACKNOWLEDGEMENTS This work was supported by grants Australian Research Council (DP200100364, DP190100039, DE160100843) and ANU Major Equipment grant (15MEC36, 16MEC26). REFERENCES [1] J.W. Dobrucki, et al. Biophys. J., vol. 93, no.5, pp. 1778-1786, 2007. [2] Y. Zheng, et al. Biophys. J., vol. 120, no. 5, pp.791-804, 2021 CONTACT Yujie Zheng; email: yujie.zheng@anu.eud.auen
dc.description.statusPeer-revieweden
dc.identifier.isbn9781713855736en
dc.identifier.isbn9781733419031en
dc.identifier.otherORCID:/0000-0002-3912-6095/work/167893726en
dc.identifier.otherORCID:/0000-0001-9453-9688/work/167894531en
dc.identifier.scopus85136935655en
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85136935655&partnerID=8YFLogxKen
dc.identifier.urihttps://www.proceedings.com/64786.htmlen
dc.identifier.urihttps://hdl.handle.net/1885/733764579
dc.language.isoenen
dc.publisherChemical and Biological Microsystems Societyen
dc.relation.ispartofMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciencesen
dc.relation.ispartofseries25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021en
dc.rightsPublisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.en
dc.subjectinterferometryen
dc.subjectlabel - freeen
dc.subjectmicrofluidicsen
dc.subjectPlateleten
dc.subjectreceptor sheddingen
dc.subjectscatteringen
dc.subjectthrombusen
dc.titleQuantification of Thrombus Kinetics Through Inhibiting Receptor Shedding with Label-Free Imaging Flow Assayen
dc.typeConference paperen
dspace.entity.typePublicationen
local.bibliographicCitation.lastpage1534en
local.bibliographicCitation.startpage1533en
local.contributor.affiliationZheng, Yujie; John Curtin School of Medical Research, ANU College of Science and Medicine, The Australian National Universityen
local.contributor.affiliationMontague, Samantha J.; John Curtin School of Medical Research, ANU College of Science and Medicine, The Australian National Universityen
local.contributor.affiliationLim, Yean J.; John Curtin School of Medical Research, ANU College of Science and Medicine, The Australian National Universityen
local.contributor.affiliationGardiner, Elizabeth E.; John Curtin School of Medical Research, ANU College of Science and Medicine, The Australian National Universityen
local.contributor.affiliationLee, Woei Ming; John Curtin School of Medical Research, ANU College of Science and Medicine, The Australian National Universityen
local.identifier.ariespublicationa383154xPUB39318en
local.identifier.pure82a70079-86c0-4cb2-abb0-fd25fac114b5en
local.identifier.urlhttps://www.proceedings.com/64786.htmlen
local.identifier.urlhttps://www.scopus.com/pages/publications/85136935655en
local.type.statusPublisheden

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